anti rabbit igg horseradish peroxidase hrp linked antibody (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti rabbit igg horseradish peroxidase hrp linked antibody
Anti Rabbit Igg Horseradish Peroxidase Hrp Linked Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 18099 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+igg+antibody/Anti-rabbit+IgG/pmc12999298-65-23-30
Average 99 stars, based on 18099 article reviews
Anti Rabbit Igg Horseradish Peroxidase Hrp Linked Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 18099 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+igg+antibody/Anti-rabbit+IgG/pmc12999298-65-23-30
Average 99 stars, based on 18099 article reviews
anti rabbit igg horseradish peroxidase hrp linked antibody - by Bioz Stars,
2026-09
99/100 stars
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Immunoprecipitation:Article Title: Ectopic CD11c Drives SMAD3-Mediated Aberrant Antigen Presentation and Epithelial–Mesenchymal Transition in Esophageal Squamous Cell Carcinoma Article Snippet: We used SimpleChIP Plus Sonication Chromatin IP Kit (Cell Signaling Technology, Cat. #56383) to carry out chromatin immunoprecipitation. .. Human esophageal squamous cell HET-1A and ESCC cells (KYSE30 and KYSE150) were fixed and cross-linked with formaldehyde before immunoprecipitation with p-SMAD3 antibody (Cell Signaling Technology, RRID: AB_2193207) or Article Title: Loss of Ezh2 promotes M2-like macrophage polarization in hepatocellular carcinoma Article Snippet: ChIP was carried out using SimpleChIP Enzymatic Chromatin IP Kit (Cat. 9003, Cell Signaling Technology) following the manufacturer’s instruction. .. Briefly, BMDMs were crosslinked with 1% formaldehyde to stabilize protein-DNA complexes, and chromatin fragments were immunoprecipitated using a rabbit anti-H3K27me3 antibody (Cat. 9733, Cell Signaling Technology) or the kit-provided normal Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules. Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with Incubation:Article Title: Combined assessment of ERO1A expression and CD163 + tumor-associated macrophage infiltration is superior to traditional assessment methods in predicting clear cell renal cell carcinoma prognosis. Article Snippet: .. The slides were incubated with the following primary antibodies at 4 °C overnight: rabbit anti-ERO1A antibody (1:100; ab177156; Abcam), Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules. Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with Article Title: Combined assessment of ERO1A expression and CD163 + tumor-associated macrophage infiltration is superior to traditional assessment methods in predicting clear cell renal cell carcinoma prognosis Article Snippet: .. The slides were incubated with the following primary antibodies at 4 °C overnight: rabbit anti-ERO1A antibody (1:100; ab177156; Abcam), Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with ChIP-qPCR:Article Title: Loss of Ezh2 promotes M2-like macrophage polarization in hepatocellular carcinoma Article Snippet: ChIP was carried out using SimpleChIP Enzymatic Chromatin IP Kit (Cat. 9003, Cell Signaling Technology) following the manufacturer’s instruction. .. Briefly, BMDMs were crosslinked with 1% formaldehyde to stabilize protein-DNA complexes, and chromatin fragments were immunoprecipitated using a rabbit anti-H3K27me3 antibody (Cat. 9733, Cell Signaling Technology) or the kit-provided normal Lysis:Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules. Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with Control:Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules. Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with Magnetic Beads:Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules. Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with Negative Control:Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules. Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with Article Title: Hypofractionated Radiotherapy Induces ISG15 + MHC-I + Neutrophils to Augment Anti-Tumor Immunity and Prime Immune Checkpoint Blockade Responses in Rectal Cancer. Article Snippet: Immunoprecipitation was performed using the appropriate antibodies as follows: p65 (ab32536, Abcam, 1:50 dilution) were used for ChIP assay. .. Mouse IgG antibody (#61656S, Cell Signaling Technology, 1:1000 dilution) and Article Title: Hepatocyte TIA1 constrains metabolic steatohepatitis by translationally suppressing Srebf1 mRNA in stress granules Article Snippet: RIP assays were conducted with stringent RNase protection using anti-TIA1 antibody (Abcam ab263945) in PA- or BSA-treated AML12 cells. .. Following lysis in polysome buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM DTT, RNaseOUT) supplemented with protease inhibitors, lysates were divided: 10% preserved as Input control, 80% immunoprecipitated with 5 μg anti-TIA1-protein A/G magnetic beads (1 h 4 °C rotation), and 10% incubated with other:Article Title: Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex. Article Snippet: Article Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex Highlights • SST and PV interneurons in the PL differentially regulate methamphetamine craving • LHGABA → PLSST and AMGlu → PLPV circuits drive stagespecific craving • KCNC2 upregulation in interneurons underlies distinct neuronal excitability changes • Specific knockdown of KCNC2 reduces craving during early and prolonged withdrawal Authors Sai Shi, Yi-Wen Sun, Jin-Jun Ding, ..., Fang Liu, Ti-Fei Yuan, Min Zhao Correspondence fang.liu@camh.ca (F.L.), ytf0707@126.com (T..-F.Y.), drminzhao@smhc.org.cn (M.Z.). In brief Shi et al. reveal that two distinct interneurons in the PL control the incubation of methamphetamine craving during different withdrawal stages.. They uncover a circuit-switch principle and propose a key molecular mediator, offering phase-specific therapeutic strategies to reduce relapse. |